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trnzol universal total rna extraction reagent  (tiangen biotech co)


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    tiangen biotech co trnzol universal total rna extraction reagent
    Trnzol Universal Total Rna Extraction Reagent, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 98/100, based on 1488 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+extraction+reagent/TRNzol+Universal+Reagent/pm42810240-74-22-34
    Average 98 stars, based on 1488 article reviews
    trnzol universal total rna extraction reagent - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Expressing:

    Article Title: HB52-PUT2 Module-Mediated Polyamine Shoot-to-Root Movement Regulates Salt Stress Tolerance in Tomato.
    Article Snippet: .. To analyze the expression of salinity tolerance‐related genes, plant total RNA was isolated by an RNA extraction reagent (Tiangen, DP419) and reverse‐transcribed with the HiScriptTM qRT SuperMix for qPCR (+gDNA wiper) kit (Vazyme, #R323). .. Real‐time PCR was then performed on an ABI VII7 real‐time PCR system (Applied Biosystems, Waltham, MA, USA), with each reaction containing ten ng first‐strand cDNA 100 ng, ten mmol L‐1 gene‐specific primers, and 12.5 μL of real‐time ChamQ SYBR Color qPCR Master Mix (Vazyme, Q421‐02).

    Article Title: HB52‐PUT2 Module‐Mediated Polyamine Shoot‐to‐Root Movement Regulates Salt Stress Tolerance in Tomato
    Article Snippet: .. To analyze the expression of salinity tolerance‐related genes, plant total RNA was isolated by an RNA extraction reagent (Tiangen, DP419) and reverse‐transcribed with the HiScriptTM qRT SuperMix for qPCR (+gDNA wiper) kit (Vazyme, #R323). .. Real‐time PCR was then performed on an ABI VII7 real‐time PCR system (Applied Biosystems, Waltham, MA, USA), with each reaction containing ten ng first‐strand cDNA 100 ng, ten mmol L ‐1 gene‐specific primers, and 12.5 μL of real‐time ChamQ SYBR Color qPCR Master Mix (Vazyme, Q421‐02).

    Isolation:

    Article Title: HB52-PUT2 Module-Mediated Polyamine Shoot-to-Root Movement Regulates Salt Stress Tolerance in Tomato.
    Article Snippet: .. To analyze the expression of salinity tolerance‐related genes, plant total RNA was isolated by an RNA extraction reagent (Tiangen, DP419) and reverse‐transcribed with the HiScriptTM qRT SuperMix for qPCR (+gDNA wiper) kit (Vazyme, #R323). .. Real‐time PCR was then performed on an ABI VII7 real‐time PCR system (Applied Biosystems, Waltham, MA, USA), with each reaction containing ten ng first‐strand cDNA 100 ng, ten mmol L‐1 gene‐specific primers, and 12.5 μL of real‐time ChamQ SYBR Color qPCR Master Mix (Vazyme, Q421‐02).

    Article Title: HB52‐PUT2 Module‐Mediated Polyamine Shoot‐to‐Root Movement Regulates Salt Stress Tolerance in Tomato
    Article Snippet: .. To analyze the expression of salinity tolerance‐related genes, plant total RNA was isolated by an RNA extraction reagent (Tiangen, DP419) and reverse‐transcribed with the HiScriptTM qRT SuperMix for qPCR (+gDNA wiper) kit (Vazyme, #R323). .. Real‐time PCR was then performed on an ABI VII7 real‐time PCR system (Applied Biosystems, Waltham, MA, USA), with each reaction containing ten ng first‐strand cDNA 100 ng, ten mmol L ‐1 gene‐specific primers, and 12.5 μL of real‐time ChamQ SYBR Color qPCR Master Mix (Vazyme, Q421‐02).

    RNA Extraction:

    Article Title: HB52-PUT2 Module-Mediated Polyamine Shoot-to-Root Movement Regulates Salt Stress Tolerance in Tomato.
    Article Snippet: .. To analyze the expression of salinity tolerance‐related genes, plant total RNA was isolated by an RNA extraction reagent (Tiangen, DP419) and reverse‐transcribed with the HiScriptTM qRT SuperMix for qPCR (+gDNA wiper) kit (Vazyme, #R323). .. Real‐time PCR was then performed on an ABI VII7 real‐time PCR system (Applied Biosystems, Waltham, MA, USA), with each reaction containing ten ng first‐strand cDNA 100 ng, ten mmol L‐1 gene‐specific primers, and 12.5 μL of real‐time ChamQ SYBR Color qPCR Master Mix (Vazyme, Q421‐02).

    Article Title: HB52‐PUT2 Module‐Mediated Polyamine Shoot‐to‐Root Movement Regulates Salt Stress Tolerance in Tomato
    Article Snippet: .. To analyze the expression of salinity tolerance‐related genes, plant total RNA was isolated by an RNA extraction reagent (Tiangen, DP419) and reverse‐transcribed with the HiScriptTM qRT SuperMix for qPCR (+gDNA wiper) kit (Vazyme, #R323). .. Real‐time PCR was then performed on an ABI VII7 real‐time PCR system (Applied Biosystems, Waltham, MA, USA), with each reaction containing ten ng first‐strand cDNA 100 ng, ten mmol L ‐1 gene‐specific primers, and 12.5 μL of real‐time ChamQ SYBR Color qPCR Master Mix (Vazyme, Q421‐02).

    Article Title: Regulatory mechanisms of the Hippo/YAP axis by G-protein coupled estrogen receptor in gastric signet-ring cell carcinoma.
    Article Snippet: .. The total RNA extraction reagent (#DP451, TianGen) was used to isolate RNA from the sample. .. RNA integrity and purity were evaluated with a NanoDrop spectrophotometer from Thermo Fisher.

    Article Title: Fat mass and obesity-associated protein downregulation trigger the activation of the sirtuin 1/forkhead box O1 signaling pathway, drive glycolysis, and promote the progression of renal cell carcinoma
    Article Snippet: .. RNA was extracted from the cells using an RNA extraction reagent (DP420, Tiangen, Beijing, China), and its purity and concentration were measured. .. The RNA was reverse-transcribed into complementary DNA (cDNA) using a cDNA synthesis kit (KR108, Tiangen, Beijing, China).

    Article Title: Potential effects of bexarotene on neural development and function in zebrafish embryos.
    Article Snippet: Dimethyl sulfoxide (DMSO, CAS No: 67–68–5) was purchased from Sangon Biotech, Shanghai, China. .. Total RNA extraction reagent (Trizol Universal, DP424), RNA reverse transcription kit (FastKing One-Step Genomic cDNA First-Strand Synthesis Premix, KR118), and quantitative reverse transcription polymerase chain reaction kit (SuperReal PreMix Plus, SYBR Green, FP205) were all purchased from Tiangen Biotech (Beijing, China). ..

    Article Title: Alterations of retinal autophagy after a blast simulated microgravity in rats.
    Article Snippet: Emerging research has confirmed the crucial role of autophagy, an endogenous repair mechanism, in various blast injuries.. However, its role in explosive ocular injury (EOI) under microgravity (MG) and normal gravity (NG) environments remains poorly understood.. Therefore, this study aimed to investigate the changes in retinal lesions and retinal autophagy over time following EOI under both NG and MG environments.

    Article Title: Sevoflurane induces Ca 2+ overload and EFHD1 upregulation, driving pyroptosis in SCLC cells
    Article Snippet: RPMI-1640 complete medium (Punosai Life Sciences, Wuhan, China, PM150110B); 0.25% trypsin solution (with EDTA and phenol red, dissolved in PBS) (Punosai Life Sciences, Wuhan, China, PB180229 ). .. Total RNA extraction reagent (TIANGEN Biotech, Beijing, China, DP405-02); reverse transcription kit (Allsheng Biotechnology, Beijing, China, AH411-02), and SYBR Green fluorescence quantification kit (TaKaRa, RR82LR). .. Primary antibodies: EFHD1 (Bioss, Beijing, China, bs-9015R), cleaved caspase 1 (Affinity, AF4005), and GSDMD (Affinity, AF4012), β-actin (Affinity, T0022); secondary antibodies: HRP-labeled goat anti-mouse IgG (Tiandeyue, Beijing, China, S001F), and HRP-labeled goat anti-rabbit IgG (Tiandeyue, Beijing, China, S004F).

    Article Title: Regulatory mechanisms of the Hippo/YAP axis by G-protein coupled estrogen receptor in gastric signet-ring cell carcinoma
    Article Snippet: .. The total RNA extraction reagent (#DP451, TianGen) was used to isolate RNA from the sample. .. RNA integrity and purity were evaluated with a NanoDrop spectrophotometer from Thermo Fisher.

    Reverse Transcription:

    Article Title: HB52-PUT2 Module-Mediated Polyamine Shoot-to-Root Movement Regulates Salt Stress Tolerance in Tomato.
    Article Snippet: .. To analyze the expression of salinity tolerance‐related genes, plant total RNA was isolated by an RNA extraction reagent (Tiangen, DP419) and reverse‐transcribed with the HiScriptTM qRT SuperMix for qPCR (+gDNA wiper) kit (Vazyme, #R323). .. Real‐time PCR was then performed on an ABI VII7 real‐time PCR system (Applied Biosystems, Waltham, MA, USA), with each reaction containing ten ng first‐strand cDNA 100 ng, ten mmol L‐1 gene‐specific primers, and 12.5 μL of real‐time ChamQ SYBR Color qPCR Master Mix (Vazyme, Q421‐02).

    Article Title: HB52‐PUT2 Module‐Mediated Polyamine Shoot‐to‐Root Movement Regulates Salt Stress Tolerance in Tomato
    Article Snippet: .. To analyze the expression of salinity tolerance‐related genes, plant total RNA was isolated by an RNA extraction reagent (Tiangen, DP419) and reverse‐transcribed with the HiScriptTM qRT SuperMix for qPCR (+gDNA wiper) kit (Vazyme, #R323). .. Real‐time PCR was then performed on an ABI VII7 real‐time PCR system (Applied Biosystems, Waltham, MA, USA), with each reaction containing ten ng first‐strand cDNA 100 ng, ten mmol L ‐1 gene‐specific primers, and 12.5 μL of real‐time ChamQ SYBR Color qPCR Master Mix (Vazyme, Q421‐02).

    Article Title: Potential effects of bexarotene on neural development and function in zebrafish embryos.
    Article Snippet: Dimethyl sulfoxide (DMSO, CAS No: 67–68–5) was purchased from Sangon Biotech, Shanghai, China. .. Total RNA extraction reagent (Trizol Universal, DP424), RNA reverse transcription kit (FastKing One-Step Genomic cDNA First-Strand Synthesis Premix, KR118), and quantitative reverse transcription polymerase chain reaction kit (SuperReal PreMix Plus, SYBR Green, FP205) were all purchased from Tiangen Biotech (Beijing, China). ..

    Article Title: Sevoflurane induces Ca 2+ overload and EFHD1 upregulation, driving pyroptosis in SCLC cells
    Article Snippet: RPMI-1640 complete medium (Punosai Life Sciences, Wuhan, China, PM150110B); 0.25% trypsin solution (with EDTA and phenol red, dissolved in PBS) (Punosai Life Sciences, Wuhan, China, PB180229 ). .. Total RNA extraction reagent (TIANGEN Biotech, Beijing, China, DP405-02); reverse transcription kit (Allsheng Biotechnology, Beijing, China, AH411-02), and SYBR Green fluorescence quantification kit (TaKaRa, RR82LR). .. Primary antibodies: EFHD1 (Bioss, Beijing, China, bs-9015R), cleaved caspase 1 (Affinity, AF4005), and GSDMD (Affinity, AF4012), β-actin (Affinity, T0022); secondary antibodies: HRP-labeled goat anti-mouse IgG (Tiandeyue, Beijing, China, S001F), and HRP-labeled goat anti-rabbit IgG (Tiandeyue, Beijing, China, S004F).

    Real-time Polymerase Chain Reaction:

    Article Title: HB52-PUT2 Module-Mediated Polyamine Shoot-to-Root Movement Regulates Salt Stress Tolerance in Tomato.
    Article Snippet: .. To analyze the expression of salinity tolerance‐related genes, plant total RNA was isolated by an RNA extraction reagent (Tiangen, DP419) and reverse‐transcribed with the HiScriptTM qRT SuperMix for qPCR (+gDNA wiper) kit (Vazyme, #R323). .. Real‐time PCR was then performed on an ABI VII7 real‐time PCR system (Applied Biosystems, Waltham, MA, USA), with each reaction containing ten ng first‐strand cDNA 100 ng, ten mmol L‐1 gene‐specific primers, and 12.5 μL of real‐time ChamQ SYBR Color qPCR Master Mix (Vazyme, Q421‐02).

    Article Title: HB52‐PUT2 Module‐Mediated Polyamine Shoot‐to‐Root Movement Regulates Salt Stress Tolerance in Tomato
    Article Snippet: .. To analyze the expression of salinity tolerance‐related genes, plant total RNA was isolated by an RNA extraction reagent (Tiangen, DP419) and reverse‐transcribed with the HiScriptTM qRT SuperMix for qPCR (+gDNA wiper) kit (Vazyme, #R323). .. Real‐time PCR was then performed on an ABI VII7 real‐time PCR system (Applied Biosystems, Waltham, MA, USA), with each reaction containing ten ng first‐strand cDNA 100 ng, ten mmol L ‐1 gene‐specific primers, and 12.5 μL of real‐time ChamQ SYBR Color qPCR Master Mix (Vazyme, Q421‐02).

    Concentration Assay:

    Article Title: Fat mass and obesity-associated protein downregulation trigger the activation of the sirtuin 1/forkhead box O1 signaling pathway, drive glycolysis, and promote the progression of renal cell carcinoma
    Article Snippet: .. RNA was extracted from the cells using an RNA extraction reagent (DP420, Tiangen, Beijing, China), and its purity and concentration were measured. .. The RNA was reverse-transcribed into complementary DNA (cDNA) using a cDNA synthesis kit (KR108, Tiangen, Beijing, China).

    Polymerase Chain Reaction:

    Article Title: Potential effects of bexarotene on neural development and function in zebrafish embryos.
    Article Snippet: Dimethyl sulfoxide (DMSO, CAS No: 67–68–5) was purchased from Sangon Biotech, Shanghai, China. .. Total RNA extraction reagent (Trizol Universal, DP424), RNA reverse transcription kit (FastKing One-Step Genomic cDNA First-Strand Synthesis Premix, KR118), and quantitative reverse transcription polymerase chain reaction kit (SuperReal PreMix Plus, SYBR Green, FP205) were all purchased from Tiangen Biotech (Beijing, China). ..

    SYBR Green Assay:

    Article Title: Potential effects of bexarotene on neural development and function in zebrafish embryos.
    Article Snippet: Dimethyl sulfoxide (DMSO, CAS No: 67–68–5) was purchased from Sangon Biotech, Shanghai, China. .. Total RNA extraction reagent (Trizol Universal, DP424), RNA reverse transcription kit (FastKing One-Step Genomic cDNA First-Strand Synthesis Premix, KR118), and quantitative reverse transcription polymerase chain reaction kit (SuperReal PreMix Plus, SYBR Green, FP205) were all purchased from Tiangen Biotech (Beijing, China). ..

    Article Title: Sevoflurane induces Ca 2+ overload and EFHD1 upregulation, driving pyroptosis in SCLC cells
    Article Snippet: RPMI-1640 complete medium (Punosai Life Sciences, Wuhan, China, PM150110B); 0.25% trypsin solution (with EDTA and phenol red, dissolved in PBS) (Punosai Life Sciences, Wuhan, China, PB180229 ). .. Total RNA extraction reagent (TIANGEN Biotech, Beijing, China, DP405-02); reverse transcription kit (Allsheng Biotechnology, Beijing, China, AH411-02), and SYBR Green fluorescence quantification kit (TaKaRa, RR82LR). .. Primary antibodies: EFHD1 (Bioss, Beijing, China, bs-9015R), cleaved caspase 1 (Affinity, AF4005), and GSDMD (Affinity, AF4012), β-actin (Affinity, T0022); secondary antibodies: HRP-labeled goat anti-mouse IgG (Tiandeyue, Beijing, China, S001F), and HRP-labeled goat anti-rabbit IgG (Tiandeyue, Beijing, China, S004F).

    Fluorescence:

    Article Title: Sevoflurane induces Ca 2+ overload and EFHD1 upregulation, driving pyroptosis in SCLC cells
    Article Snippet: RPMI-1640 complete medium (Punosai Life Sciences, Wuhan, China, PM150110B); 0.25% trypsin solution (with EDTA and phenol red, dissolved in PBS) (Punosai Life Sciences, Wuhan, China, PB180229 ). .. Total RNA extraction reagent (TIANGEN Biotech, Beijing, China, DP405-02); reverse transcription kit (Allsheng Biotechnology, Beijing, China, AH411-02), and SYBR Green fluorescence quantification kit (TaKaRa, RR82LR). .. Primary antibodies: EFHD1 (Bioss, Beijing, China, bs-9015R), cleaved caspase 1 (Affinity, AF4005), and GSDMD (Affinity, AF4012), β-actin (Affinity, T0022); secondary antibodies: HRP-labeled goat anti-mouse IgG (Tiandeyue, Beijing, China, S001F), and HRP-labeled goat anti-rabbit IgG (Tiandeyue, Beijing, China, S004F).



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